Molarity, Mass & Volume Online Calculator — Solve for Concentration, Volume, or Mass
The core relationship used in solution preparation:
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)
Know any two of the three variables and solve for the third. The tool handles unit conversion automatically — no more counting decimal places.
Common pitfalls:
- Use the molecular weight for the correct salt form. Many reagents are supplied as hydrochlorides, sodium salts, or hydrates — their formula weights differ substantially from the free base. Always read the FW from the bottle label, not from a database entry for the parent compound.
- Hydrated reagents (e.g., CuSO₄·5H₂O) must use the hydrated formula weight; using the anhydrous MW gives a lower actual concentration than intended.
- Solubility limits. A calculation says you can make it — that doesn’t mean it will dissolve. DMSO stock solutions are typically 10–100 mM; for poorly water-soluble compounds, check solubility before you start.
- Proteins and antibodies are typically specified in mg/mL, not mol/L. Converting requires an accurate molecular weight; glycosylation can make the actual MW higher than the theoretical value.
FAQ
Why is the prepared concentration off despite following the calculation?
The most common cause is using the wrong molecular weight. When the reagent is a hydrochloride, sodium salt, or hydrate, its actual formula weight differs from that of the parent compound. Always use the FW printed on the bottle label.
How do I convert a protein concentration from mg/mL to M?
You need an accurate molecular weight. For recombinant proteins, the theoretical MW can be calculated from the amino acid sequence, but the actual MW of glycosylated proteins will be higher than this theoretical value.
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