Statistics Calculators
The hard part here is never the computation — it is the choice.
If you do not know which test applies, start with the statistical test chooser: walk through data type, number of groups and pairing, and it tells you where you land and why.
Three mistakes dominate, each with a tool that addresses it:
- Not correcting for multiple comparisons. Run 20 tests and one comes back p < 0.05 by luck alone. FDR / Benjamini-Hochberg handles this; in omics data it is not optional.
- Using a t-test on data that is not normal. With small samples the normality test itself has little power, which is exactly when non-parametric tests are the safer choice.
- Reading correlation as effect. Correlation coefficients quantify co-variation — Pearson captures linear, Spearman monotone — and R² = 0.9 implies nothing about importance. High R² is especially cheap when n is small.
One thing belongs before the experiment: sample size estimation. Power computed after the fact tells you nothing; a sample size fixed in advance does.
The omics-facing tools are listed separately: log₂FC, enrichment testing, RNA-seq normalisation and ΔΔCt. The principles above still apply — only the context changes.
Statistics
Multiple Testing Correction Calculator: BH (FDR), BY, and Bonferroni
Paste a column of p-values and get BH q-values, BY, and Bonferroni corrections side by side in one step.
qPCR 2^−ΔΔCt Relative Quantification Online Calculator
Paste your Ct table and get ΔCt, ΔΔCt, fold-change, and within-group dispersion calculated automatically.
Gene List Intersection, Union & Difference — Online Venn Set Calculator
Paste 2–3 gene/ID lists and instantly get intersections, unions, and unique elements, with deduplication and case normalization.
log2FC ↔ Fold Change Converter
Convert between log2FC and fold change, with percent regulation and a common threshold reference table.
Standard Curve Linear Regression and Back-Calculation of Unknown Samples (BCA / Bradford / ELISA)
Paste standard concentrations and absorbance readings to obtain slope, intercept, and R², then back-calculate unknown sample concentrations.
Sequencing Depth & Data Volume Calculator (Lander-Waterman)
Convert between sequencing depth, read count, data volume, and genome size — in either direction.
Sample Size Estimation for Two-Group Comparison (t-test / Proportion Test)
Estimate the required sample size per group from effect size, significance level, and statistical power.
Community Diversity Index Calculator (Shannon / Simpson / Chao1)
Paste a species abundance table and compute Shannon, Simpson, evenness, and Chao1 richness estimates.
Enrichment Significance Test Calculator — Hypergeometric Distribution / Fisher's Exact Test
Enter background gene count, pathway gene count, list size, and overlap to compute p-value, fold enrichment, and expected overlap.
qPCR Amplification Efficiency Calculator — Standard Curve Slope to E, Pfaffl Relative Quantification
Fit a standard curve from serial dilutions to calculate amplification efficiency E and R²; apply the Pfaffl method for efficiency-corrected relative quantification.
RNA-seq Expression Normalization Calculator: RPKM / FPKM / TPM Converter
Paste gene read counts and lengths to compute RPKM / FPKM and TPM at once — and see directly that TPM sums to 1,000,000 while RPKM does not.
Online t-Test Calculator: Independent Samples / Paired Samples / One-Sample (with Welch Correction)
Paste two data sets to get the t-statistic, degrees of freedom, two-tailed p-value, mean-difference confidence interval, and Cohen's d — Welch correction on by default.
One-Way ANOVA Calculator Online (Post-Hoc Tests & Multiple Comparison Correction)
Compare means across three or more groups: F statistic, ANOVA table, p-value, η², and optional pairwise post-hoc tests with Bonferroni or BH correction.
Mann-Whitney U and Wilcoxon Signed-Rank Test Calculator
Two-group comparison for skewed or ordinal data: computes U/W statistics, exact or approximate p-values, effect size, and the minimum achievable p for your sample size.
Chi-Square and Fisher's Exact Test Calculator (with OR / RR)
Contingency table independence tests — Pearson chi-square, Yates' correction, and Fisher's exact p-value; OR and RR with confidence intervals for 2×2 tables.
Correlation Coefficient Calculator Online: Pearson, Spearman, and Kendall
Compute all three correlation coefficients with p-values in one step, plus Pearson's 95% CI and r², with automatic warnings for outliers or non-linearity.
How to Choose a Statistical Test: By Data Type, Number of Groups, and Paired vs. Independent Design
Answer three questions to identify the right test: comparing values or proportions, how many groups, paired or independent. Each row links to the corresponding tool.
Michaelis-Menten Km and Vmax Calculator (Nonlinear Fit + Three Linearization Methods)
Nonlinear fit for Km and Vmax with Lineweaver-Burk, Hanes-Woolf, and Eadie-Hofstee linearizations shown in parallel—their spread directly reports data quality.
IC50 / EC50 Four-Parameter Logistic Fit Calculator (with Data Reliability Checks)
Fit IC50/EC50 and Hill slope from concentration–response data using 4PL; reports plateau coverage and how much fixing the slope to 1 shifts the result.
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